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beads affigel 10 gel  (Bio-Rad)


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    Structured Review

    Bio-Rad beads affigel 10 gel
    Beads Affigel 10 Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affigel-10+beads/Affi-Gel+10+Gel/pmc12913626-259-12-13
    Average 99 stars, based on 2203 article reviews
    beads affigel 10 gel - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: Antibodies SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: The Short Arm of Laminin ?2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin ?4 Chain
    Article Snippet: Membrane proteins of EJ-1 cells were collected according to the method of Hoffman et al. (1998) and then incubated with a γ2sa-conjugated beads, which had been prepared by binding the purified γ2sa to Affigel-10 beads (Bio-Rad, Hercules, CA), in PBS buffer at 4°C overnight.

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Peptide pulldown Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Cellular cholesterol directly activates Smoothened in Hedgehog signaling
    Article Snippet: Affigel-10 beads (BioRad) were converted to primary amine beads, by reaction with 4,7,10-trioxa-1,13-tridecanediamine (0.5 M in dry isopropanol), for 3 hours at room temperature.

    Article Title: A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats
    Article Snippet: Anti-FXI monoclonal IgG 14E11 was linked to Affigel-10 beads (BioRad, Richmond, CA, USA) at 3 mg/mL [ ].

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Protein misfolding specifies recruitment to cytoplasmic inclusion bodies
    Article Snippet: Lysates were rotated at 4°C for 15 min and cleared by centrifugation at 20,000 g for 20 min. 0.5 mg of cleared lysate was diluted fivefold with cold binding buffer containing 10 mM Hepes, 20 mM imidazole, 150 mM NaCl, and 0.5% Triton X-100, pH 7.4, and added to 25 μl Affigel-10 beads (Bio-Rad Laboratories) covalently conjugated to purified hplic2 UBA prepared as a 50% slurry in binding buffer.

    Binding Assay:

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: Antibodies SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: The Short Arm of Laminin ?2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin ?4 Chain
    Article Snippet: Membrane proteins of EJ-1 cells were collected according to the method of Hoffman et al. (1998) and then incubated with a γ2sa-conjugated beads, which had been prepared by binding the purified γ2sa to Affigel-10 beads (Bio-Rad, Hercules, CA), in PBS buffer at 4°C overnight.

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Peptide pulldown Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Cellular cholesterol directly activates Smoothened in Hedgehog signaling
    Article Snippet: Affigel-10 beads (BioRad) were converted to primary amine beads, by reaction with 4,7,10-trioxa-1,13-tridecanediamine (0.5 M in dry isopropanol), for 3 hours at room temperature.

    Article Title: A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats
    Article Snippet: Anti-FXI monoclonal IgG 14E11 was linked to Affigel-10 beads (BioRad, Richmond, CA, USA) at 3 mg/mL [ ].

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Protein misfolding specifies recruitment to cytoplasmic inclusion bodies
    Article Snippet: Lysates were rotated at 4°C for 15 min and cleared by centrifugation at 20,000 g for 20 min. 0.5 mg of cleared lysate was diluted fivefold with cold binding buffer containing 10 mM Hepes, 20 mM imidazole, 150 mM NaCl, and 0.5% Triton X-100, pH 7.4, and added to 25 μl Affigel-10 beads (Bio-Rad Laboratories) covalently conjugated to purified hplic2 UBA prepared as a 50% slurry in binding buffer.

    Purification:

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: Antibodies SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: The Short Arm of Laminin ?2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin ?4 Chain
    Article Snippet: Membrane proteins of EJ-1 cells were collected according to the method of Hoffman et al. (1998) and then incubated with a γ2sa-conjugated beads, which had been prepared by binding the purified γ2sa to Affigel-10 beads (Bio-Rad, Hercules, CA), in PBS buffer at 4°C overnight.

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Peptide pulldown Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Cellular cholesterol directly activates Smoothened in Hedgehog signaling
    Article Snippet: Affigel-10 beads (BioRad) were converted to primary amine beads, by reaction with 4,7,10-trioxa-1,13-tridecanediamine (0.5 M in dry isopropanol), for 3 hours at room temperature.

    Article Title: A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats
    Article Snippet: Anti-FXI monoclonal IgG 14E11 was linked to Affigel-10 beads (BioRad, Richmond, CA, USA) at 3 mg/mL [ ].

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Protein misfolding specifies recruitment to cytoplasmic inclusion bodies
    Article Snippet: Lysates were rotated at 4°C for 15 min and cleared by centrifugation at 20,000 g for 20 min. 0.5 mg of cleared lysate was diluted fivefold with cold binding buffer containing 10 mM Hepes, 20 mM imidazole, 150 mM NaCl, and 0.5% Triton X-100, pH 7.4, and added to 25 μl Affigel-10 beads (Bio-Rad Laboratories) covalently conjugated to purified hplic2 UBA prepared as a 50% slurry in binding buffer.

    Incubation:

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: Antibodies SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: The Short Arm of Laminin ?2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin ?4 Chain
    Article Snippet: Membrane proteins of EJ-1 cells were collected according to the method of Hoffman et al. (1998) and then incubated with a γ2sa-conjugated beads, which had been prepared by binding the purified γ2sa to Affigel-10 beads (Bio-Rad, Hercules, CA), in PBS buffer at 4°C overnight.

    Article Title: IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
    Article Snippet: SUMO-2/3 monoclonal antibody (8A2) [ 9 ] was purified from mouse ascites fluid as described [ 12 ] and immobilized on Affigel-10 beads (BioRad) according to the manufacturer’s protocol.

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Peptide pulldown Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Cellular cholesterol directly activates Smoothened in Hedgehog signaling
    Article Snippet: Affigel-10 beads (BioRad) were converted to primary amine beads, by reaction with 4,7,10-trioxa-1,13-tridecanediamine (0.5 M in dry isopropanol), for 3 hours at room temperature.

    Article Title: A Common Missense Variant Causing Factor XI Deficiency and Increased Bleeding Tendency in Maine Coon Cats
    Article Snippet: Anti-FXI monoclonal IgG 14E11 was linked to Affigel-10 beads (BioRad, Richmond, CA, USA) at 3 mg/mL [ ].

    Article Title: Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function
    Article Snippet: Synthetic rNR2B(845-861) preceded by a Gly-Ala spacer [H- GAGA Q 845 FRHCFMGVCSGKPG MV 861 NH 2 ] (Biomatik, Wilmington, DE) was covalently linked to AffiGel-10 beads (Bio-Rad, Hercules, CA); this sequence follows transmembrane domain 4 and is referred to as NR2B juxtamembrane domain (2B-JM).

    Article Title: Protein misfolding specifies recruitment to cytoplasmic inclusion bodies
    Article Snippet: Lysates were rotated at 4°C for 15 min and cleared by centrifugation at 20,000 g for 20 min. 0.5 mg of cleared lysate was diluted fivefold with cold binding buffer containing 10 mM Hepes, 20 mM imidazole, 150 mM NaCl, and 0.5% Triton X-100, pH 7.4, and added to 25 μl Affigel-10 beads (Bio-Rad Laboratories) covalently conjugated to purified hplic2 UBA prepared as a 50% slurry in binding buffer.



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    Image Search Results


    Journal: Advanced Science

    Article Title: Aberrant Cholesterol Metabolism and Wnt/ β ‐Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer Growth

    doi: 10.1002/advs.202200750

    Figure Lengend Snippet:

    Article Snippet: Affigel‐10 beads , Bio‐Rad , #1085.

    Techniques: Recombinant, Software